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beadbound dna protein complex  (Thermo Fisher)


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    Structured Review

    Thermo Fisher beadbound dna protein complex
    Beadbound Dna Protein Complex, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/beadbound+dna+protein+complex/Deoxyribonucleic+acid/pm27496972-60-100-109
    Average 99 stars, based on 1 article reviews
    beadbound dna protein complex - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Recombinant:

    Article Title: Zebrafish IRF1, IRF3, and IRF7 Differentially Regulate IFNΦ1 and IFNΦ3 Expression through Assembly of Homo- or Heteroprotein Complexes.
    Article Snippet: .. Zebrafish IRF proteins were in vitro–translated in the TNT SP6 Quick Coupled Transcription/Translation System supplemented with 40 mM KCl (Promega) or expressed as recombinant proteins by a prokaryotic expression system, according to previous studies (27, 28), or they were overexpressed as tag-fused proteins by transfection of HEK293T cells for 24 h. Briefly, 25 ml of individual translated products, 4 mg of recombinant products, or an adequate amount of IRF-overexpressing HEK293T cell lysates (one fifth of a 10 cm-dish, unless otherwise indicated), together with 1 mg of biotinylated promoter dsDNA, was incubated in HKMG buffer at 4 ̊C for 24 h. The beadbound DNA-protein complex was captured with M-280 streptavidin-coupled Dynabeads (Invitrogen) and washed with incubation buffer five times, followed by further Western blotting analysis. .. Tissues were sampled from healthy zebrafish or ones that had been infected for 48 h with SVCV, and total RNA was extracted for further expression analysis of zebrafish IRFs, zebrafish IFNs, and SVCV genes, including L, N, and G, by quantitative RT-PCR (qRT-PCR). qRT-PCR was also used to detect zebrafish IRF and IFN expression in ZFLs, which were seeded in sixwell plates and transfected with polyinosinic-polycytidylic acid [poly(I:C); 2 mg/ml] or treated with SVCV (1000 TCID50/ml) and recombinant crucian carp IFN (rIFN) protein (5 ng/ml). rIFN protein was generated by a prokaryotic expression system (27). qRT-PCR was performed in a DNA Engine Chromo4 Real-Time PCR System with SYBR Green Real-Time PCR Master Mix (both from Bio-Rad) (27).

    Transfection:

    Article Title: Zebrafish IRF1, IRF3, and IRF7 Differentially Regulate IFNΦ1 and IFNΦ3 Expression through Assembly of Homo- or Heteroprotein Complexes.
    Article Snippet: .. Zebrafish IRF proteins were in vitro–translated in the TNT SP6 Quick Coupled Transcription/Translation System supplemented with 40 mM KCl (Promega) or expressed as recombinant proteins by a prokaryotic expression system, according to previous studies (27, 28), or they were overexpressed as tag-fused proteins by transfection of HEK293T cells for 24 h. Briefly, 25 ml of individual translated products, 4 mg of recombinant products, or an adequate amount of IRF-overexpressing HEK293T cell lysates (one fifth of a 10 cm-dish, unless otherwise indicated), together with 1 mg of biotinylated promoter dsDNA, was incubated in HKMG buffer at 4 ̊C for 24 h. The beadbound DNA-protein complex was captured with M-280 streptavidin-coupled Dynabeads (Invitrogen) and washed with incubation buffer five times, followed by further Western blotting analysis. .. Tissues were sampled from healthy zebrafish or ones that had been infected for 48 h with SVCV, and total RNA was extracted for further expression analysis of zebrafish IRFs, zebrafish IFNs, and SVCV genes, including L, N, and G, by quantitative RT-PCR (qRT-PCR). qRT-PCR was also used to detect zebrafish IRF and IFN expression in ZFLs, which were seeded in sixwell plates and transfected with polyinosinic-polycytidylic acid [poly(I:C); 2 mg/ml] or treated with SVCV (1000 TCID50/ml) and recombinant crucian carp IFN (rIFN) protein (5 ng/ml). rIFN protein was generated by a prokaryotic expression system (27). qRT-PCR was performed in a DNA Engine Chromo4 Real-Time PCR System with SYBR Green Real-Time PCR Master Mix (both from Bio-Rad) (27).

    Incubation:

    Article Title: Zebrafish IRF1, IRF3, and IRF7 Differentially Regulate IFNΦ1 and IFNΦ3 Expression through Assembly of Homo- or Heteroprotein Complexes.
    Article Snippet: .. Zebrafish IRF proteins were in vitro–translated in the TNT SP6 Quick Coupled Transcription/Translation System supplemented with 40 mM KCl (Promega) or expressed as recombinant proteins by a prokaryotic expression system, according to previous studies (27, 28), or they were overexpressed as tag-fused proteins by transfection of HEK293T cells for 24 h. Briefly, 25 ml of individual translated products, 4 mg of recombinant products, or an adequate amount of IRF-overexpressing HEK293T cell lysates (one fifth of a 10 cm-dish, unless otherwise indicated), together with 1 mg of biotinylated promoter dsDNA, was incubated in HKMG buffer at 4 ̊C for 24 h. The beadbound DNA-protein complex was captured with M-280 streptavidin-coupled Dynabeads (Invitrogen) and washed with incubation buffer five times, followed by further Western blotting analysis. .. Tissues were sampled from healthy zebrafish or ones that had been infected for 48 h with SVCV, and total RNA was extracted for further expression analysis of zebrafish IRFs, zebrafish IFNs, and SVCV genes, including L, N, and G, by quantitative RT-PCR (qRT-PCR). qRT-PCR was also used to detect zebrafish IRF and IFN expression in ZFLs, which were seeded in sixwell plates and transfected with polyinosinic-polycytidylic acid [poly(I:C); 2 mg/ml] or treated with SVCV (1000 TCID50/ml) and recombinant crucian carp IFN (rIFN) protein (5 ng/ml). rIFN protein was generated by a prokaryotic expression system (27). qRT-PCR was performed in a DNA Engine Chromo4 Real-Time PCR System with SYBR Green Real-Time PCR Master Mix (both from Bio-Rad) (27).

    Western Blot:

    Article Title: Zebrafish IRF1, IRF3, and IRF7 Differentially Regulate IFNΦ1 and IFNΦ3 Expression through Assembly of Homo- or Heteroprotein Complexes.
    Article Snippet: .. Zebrafish IRF proteins were in vitro–translated in the TNT SP6 Quick Coupled Transcription/Translation System supplemented with 40 mM KCl (Promega) or expressed as recombinant proteins by a prokaryotic expression system, according to previous studies (27, 28), or they were overexpressed as tag-fused proteins by transfection of HEK293T cells for 24 h. Briefly, 25 ml of individual translated products, 4 mg of recombinant products, or an adequate amount of IRF-overexpressing HEK293T cell lysates (one fifth of a 10 cm-dish, unless otherwise indicated), together with 1 mg of biotinylated promoter dsDNA, was incubated in HKMG buffer at 4 ̊C for 24 h. The beadbound DNA-protein complex was captured with M-280 streptavidin-coupled Dynabeads (Invitrogen) and washed with incubation buffer five times, followed by further Western blotting analysis. .. Tissues were sampled from healthy zebrafish or ones that had been infected for 48 h with SVCV, and total RNA was extracted for further expression analysis of zebrafish IRFs, zebrafish IFNs, and SVCV genes, including L, N, and G, by quantitative RT-PCR (qRT-PCR). qRT-PCR was also used to detect zebrafish IRF and IFN expression in ZFLs, which were seeded in sixwell plates and transfected with polyinosinic-polycytidylic acid [poly(I:C); 2 mg/ml] or treated with SVCV (1000 TCID50/ml) and recombinant crucian carp IFN (rIFN) protein (5 ng/ml). rIFN protein was generated by a prokaryotic expression system (27). qRT-PCR was performed in a DNA Engine Chromo4 Real-Time PCR System with SYBR Green Real-Time PCR Master Mix (both from Bio-Rad) (27).



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    beadbound dna protein complex  (Thermo Fisher)
    99
    Thermo Fisher beadbound dna protein complex
    Beadbound Dna Protein Complex, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/beadbound+dna+protein+complex/Deoxyribonucleic+acid/pm27496972-60-100-109
    Average 99 stars, based on 1 article reviews
    beadbound dna protein complex - by Bioz Stars, 2026-09
    99/100 stars
    		  Buy from Supplier

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